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KMID : 0359320100500030179
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Korean Journal of Veterinary Research
2010 Volume.50 No. 3 p.179 ~ p.185
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Evaluation of character on lymphocyte subpopulations from peripheral blood in Hanwoo and Holstein neonatal calves
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Jung Young-Hun
Hur Tai-Young
Kang Seog-Jin
Ki Kwang-Seok
Park Sung-Jae
Lee Myeung-Sik
Suh Guk-Hyun
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Abstract
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The present study was undertaken to establish reference values for the composition blood lymphocyte populations and compare forty three Hanwoo neonatal calves (KC) with twenty one Holstein calves (HC) by blood cell count and immunophynotying. The percentages of CD2+, CD4+, CD8+, CD26+, ACT2+, MHC class, MHC class II and WC1+ T cells, B cells were determined by flow cytometry. The number of lymphocyte and monocyte in HC were higher than those of KC. However, the number of neutrophils was higher in HC than KC. The proportions of CD2+, CD4+, CD8+, MHC class, and WC1+ lymphocytes remained relatively stable during the study period, while there was a moderate increase in the relative percentage of CD26+, ACT2+, MHC class II and B cell from birth to approximately 3 weeks of age. Marked differences in the relative proportions of the lymphocyte subpopulations were noted between the individual calves. The present study shows that the T-cell subpopulations are present in peripheral blood of KC at levels comparable with HC, while the MHC class II and B cell population of KC increases significantly with age. The absolute number of WBC in KC was due to the decrease of absolute number of neutrophil rather than the increase of lymphocyte. The results indicated that KC have significantly higher number of neutrophils, and proportion of MHC class II and B cell than HC.
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KEYWORD
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flow cytometry, Hanwoo and Holstein calf, immunophynotype, lymphocyte subpopulation
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